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Microcephaly is a rare neurodevelopmental disorder characterized by a reduction in head circumference, usually affecting cognitive development. The genetic etiology is heterogeneous, ranging from monogenic to complex multigenic inheritance. Recently, we identified the microcephaly in the guinea pig associated 1 (MCA1) gene as the major genetic determinant of autosomal recessive microcephaly in the guinea pig. Here, we describe a novel genetic locus, Nap1, as the molecular basis of autosomal dominant microcephaly in an Italian family with 9 affected individuals. The observed clinical phenotype consisted of variable degrees of intellectual disability, microcephaly, and small head circumference (from 30% to 82% of individuals) with a variable degree of speech delay. Genomic DNA from each member of the family was screened for mutations in MCA1 and NAP1. Single nucleotide variation screening revealed only the known NAP1 mutation, c.2472T>C (p.F824S), in the proband. The family showed a tight linkage to the MCA1 locus on chromosome 2q22 and to the NAP1 locus on chromosome 9q33.1. To confirm the causality of the NAP1 mutation in this family, expression of the NAP1 protein in HEK293T cells was induced by transient transfection of pcDNA3.1-NAP1 using calcium phosphate. The expression of the NAP1 protein revealed by Western blot showed that the p.F824S mutation caused a substitution of phenylalanine by serine at amino acid 824. This study identifies a novel gene, NAP1, as a possible candidate for dominantly inherited microcephaly.Q: Combine multiple JsonResult into one JsonResult I have multiple JSONResult like public JsonResult Data(int ID, int What's New in the Morgan Stream Switcher V0.99? 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